The efficacy of a commercial beta-glucan preparation, EcoActiva (TM), on stimulating respiratory burst activity of head-kidney macrophages from pink snapper (Pagrus auratus), Sparidae
Mt. Cook et al., The efficacy of a commercial beta-glucan preparation, EcoActiva (TM), on stimulating respiratory burst activity of head-kidney macrophages from pink snapper (Pagrus auratus), Sparidae, FISH SHELLF, 11(8), 2001, pp. 661-672
This study investigated the in vitro effects of a commercial beta -glucan p
reparation, EcoActiva (TM), on the, respiratory burst activity of head-kidn
ey macrophages isolated from pink snapper (Pagrus auratus), a marine fish c
ultured in Australia. Macrophages incubated with EcoActival (TM) displayed
morphological characteristics of activation, and were stimulated to produce
superoxide. Pre-incubation with low levels of EcoActiva (TM) significantly
increased the response to phorbol myristate acetate (PMA) and lipopolysacc
haride (LPS), indicating that EcoActiva (TM) could prime these macrophages.
Co-culturing macrophages with both LPS and PMA, or EcoActiva (TM) and PMA,
increased burst activity compared with the response to PMA alone, however,
this increase was additive and not synergistic. These results suggest that
EcoActiva (TM) is able to stimulate non-specific immunity in snapper throu
gh increased respiratory burst activity of macrophages, an important compon
ent of the host defence network. (C) 2001 Academic Press.