A global perspective, of ground level, 'ambient' carbon dioxide for assessing the response of plants to atmospheric CO2

For most studies involving the response of plants to future concentrations of atmospheric carbon dioxide (CO2), a current concentration of 360-370 mu atm is assumed, based on recent data obtained from the Mauna Loa observator y. In the present study, average seasonal diurnal values of ambient CO2 obt ained at ground level from three global locations (Australia, Japan and the USA) indicated that the average CO2 (at canopy height) can vary from over 500 mu atm at night to 350 mu atm during the day with average 24-h values r anging from 390 to 465 mu atm. At all sites sampled, ambient CO2 rose to a maximum value during the pre-dawn period (03.00-06.00 hours); at sunrise, C O2 remained elevated for several hours before declining to a steady-state c oncentration between 350 and 400 mu atm by mid-morning (08.00-10.00 hours). Responses of plant growth to simulations of the observed variation of in s itu CO2 were compared to growth at a constant CO2 concentration in controll ed environment chambers. Three diurnal patterns were used (constant 370 mu atm CO2, constant 370 during the day (07.00-19.00 hours), high CO2 (500 mu atm) at night; or, high CO2 (500 mu atm) at night and during the early morn ing (07.00-09.00 hours) decreasing to 370 mu atm by 10.00 hours). Three pla nt species soybean (Glycine max, L (Merr.), velvetleaf (Abutilon theophrast i L.) and tomato (Lycopersicon esculentum L.) - were grown in each of these environments. For soybean, high night-time CO2 resulted in a significant i ncrease in net assimilation rate (NAR), plant growth, leaf area and biomass relative to a constant ambient value of CO2 by 29 days after sowing. Signi ficant increases in NAR for all three species, and significant increases in leaf area, growth and total biomass for two of the three C3 species tested (velvetleaf and soybean) were also observed after 29 days post sowing for the high night/early morning diurnal pattern of CO2. Data from these experi ments suggest that the ambient CO2 concentration experienced by some plants is higher than the Mauna Loa average, and that growth of some agricultural species at in situ CO2 levels can differ significantly from the constant C O2 value used as a control in many CO2 experiments. This suggests that a re assessment of control conditions used to quantify the response of plants to future, elevated CO2 may be required.