Identity and expression pattern of chemosensory proteins in Heliothis virescens (Lepidoptera, Noctuidae)

Analyzing the chemosensory organs of the moth Heliothis virescens, three pr oteins belonging to the family of insect chemosensory proteins (CSPs) have been cloned they are called HvirCSP1. HvirCSP2 and HvirCSP3. The HvirCSPs s how about 50% identity between each other and 30-76% identity to CSPs from other species. Overall. they are rather hydrophilic proteins but include a conserved hydrophobic motif. Tissue distribution and temporal expression pa ttern during the last pupal stages were assessed by Northern blots. HvirCSP mRNAs were detected in various parts of the adult body with a particular h igh expression level in legs. The expression of HvirCSP1 in legs started ea rly during adult development, in parallel with the appearance of the cuticl e. HvirCSP1 mRNA was detectable five days before eclosion (day E-5), increa sed dramatically on day E-3 and remained at high level into adult life. The tissue distribution and the time course of appearance of HvirCSPs are in a greement with a possible role in contact chemosensation. (C) 2001 Elsevier Science Ltd. All rights reserved.