CD65 THROMBOXANE B2, AND BETA-THROMBOGLOBULIN - A COMPARISON BETWEEN DIFFERENT MARKERS OF PLATELET ACTIVATION AFTER CONTACT WITH BIOMATERIALS

The authors examined the modifications of some markers of platelet act ivation after contact with biomaterials. Glycoprotein GMP-140 (CD62) w as evaluated by flow cytometry; beta-thromboglobulin (beta-TG) and thr omboxane B-2 (TXB2) were determined by radioimmunoassay. Polyethylene terephthalate (PET) induced a remarkable platelet adhesion and a signi ficant increase in beta-TG and TXB2, with no increase in CD62 on the n onadherent platelets. Pyrolytic carbon-coated PET (PC) did not induce platelet adhesion after 15 min of contact, but a significant increase in CD62 was detected. After 30 min a significant increase in platelet adhesion as well as the release of beta-TG and TXB2 were noted. The in crease was lower than that observed for uncoated PET, and after 30 min of contact with PC the increase no longer was observed. (C) 1997 John Wiley & Sons, Inc.