Activated T cells recognize Ag in the retina, an immune privileged tissue,
and may mediate autoimmune disease. In contrast, this report asks if restin
g, Ag-specific CD4(+) CD44(+) T cells can recognize Ag expressed in the ret
ina. As a probe for Ag, 3E9 T cells specific for an immunodominant epitope
of beta -galactosidase (beta -gal) were transferred to transgenic (Tg) mice
expressing beta -gal in retinal photoreceptor cells, or to ROSA26 mice whi
ch express beta -gal widely. The survival, phenotype, and responsiveness of
transferred 3E9 T cells were unaffected by the presence of retinal beta -g
al, but altered by recognition of beta -gal in the ROSA26 mice. Inoculation
or induction of activated T cells with specificity for this epitope produc
ed autoimmune uveoretinitis, showing that the retinal beta -gal is expresse
d at immunologically significant levels. We conclude that sequestration pro
vides a substantial barrier to recognition of Ag in quiet retina, and that
insufficient Ag leaves the retina for detectable immune recognition outside
of the retina. (C) 2001 Elsevier Science B.V. All rights reserved.