In vitro cytotoxicity of six dentin bonding agents

The cytotoxicity of six dentin bonding agents (Syntac, Solobond, Bond 1, Sc otchbond 1, Heliobond and F-2000) was tested against an established cell li ne, L929. Under aseptic conditions 3, 5 and 10 muL dentin bonding agents we re placed in the centre of Petri dishes. Each dish was covered with a 5-mL suspension of fibroblasts at a concentration of 40 000 cells mL(-1). The cu ltures were incubated at 37 degreesC and cytotoxicity was assessed by a qua ntitative technique at 24 and 72 h. All the dentin bonding agents were foun d to be cytotoxic. Scotchbond 1 and F-2000 showed the highest cytotoxicity followed by Solobond and Bond 1. Heliobond and Syntac were the least toxic materials.