Mr. Baker et al., Activation of cerebellar climbing fibres to rat cerebellar posterior lobe from motor cortical output pathways, J PHYSL LON, 536(3), 2001, pp. 825-839
1. The activation of climbing fibres projecting to the posterior lobe cereb
ellar cortex by focal stimulation of the cerebral corticofugal pathway was
investigated in anaesthetised rats. Large climbing fibre responses were evo
ked in parts of crus II and paramedian lobule by stimulation of corticofuga
l fibres. Lesions of the pyramidal tract just rostral to the inferior olive
substantially reduced these responses, suggesting that they were. not medi
ated by relays in the rostral brainstem.
2. By comparison of latencies of climbing fibre responses evoked from diffe
rent locations in the corticofugal pathway, the conduction velocities of th
e corticofugal fibres that mediate the responses were estimated to be 1.9 /- 0.3 m s(-1) (mean +/- The fastest conducting corticofugal fibres were es
timated to conduct significantly faster (18.7 +/- 2.3 m s(-1)).
3. Climbing fibre responses with similar form and cerebellar distribution w
ere evoked from sites in the pyramidal tract rostral and caudal to the infe
rior olive. This suggests that at least a proportion of the fibres that act
ivate climbing fibres are corticospinal fibres.
4. Lesions of the dorsal column nuclei did not affect the climbing fibre re
sponses evoked in crus II, and produced a relatively small reduction of the
responses in the paramedian lobule. This implies that the climbing fibre r
esponses were not exclusively mediated via the dorsal column nuclei.
5. Corticofugal evoked climbing fibre responses were mapped across the cere
bellar hemisphere. At some sites they were co-localised with responses evok
ed by limb afferents. On the basis of limb afferent inputs and other work,
these zones were tentatively identified as being functionally equivalent to
the c1, c2 and d zones described in the cat.