Jj. Pesek et al., Applications of chemically modified and coated etched capillaries for the separation of basic molecules, J SEP SCI, 24(9), 2001, pp. 729-735
A comparison of the migration behavior of two antibiotics is made between s
everal types of etched columns used in the open tubular format of capillary
electrochromatography. The types of columns evaluated are: 1) etched and u
nmodified; 2) etched and bonded with an octadecyl moiety; 3) etched and bon
ded with:a cholesterol moiety; 4) etched and coated with an anionic surfact
ant; and 5) etched and coated with a neutral (zwitterionic) surfactant. In
addition to evaluating the influence of the stationary phase, the effect of
buffer pH is also measured. Each of the antibiotic samples consist of seve
ral components and the resolution capabilities of the various columns are t
ested in order to determine how OTCEC can be optimized for the analysis of
small basic compounds.