Detection of Shiga-toxigenic Escherichia coli (STEC) in fresh seafood and meat marketed in Mangalore, India by PCR

Authors
Kumar, HS Otta, SK Karunasagar, I Karunasagar, I
Citation
Hs. Kumar et al., Detection of Shiga-toxigenic Escherichia coli (STEC) in fresh seafood and meat marketed in Mangalore, India by PCR, LETT APPL M, 33(5), 2001, pp. 334-338
Citations number
26
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
LETTERS IN APPLIED MICROBIOLOGY
ISSN journal
02668254 → ACNP
Volume
33
Issue
5
Year of publication
2001
Pages
334 - 338
Database
ISI
SICI code
0266-8254(200111)33:5<334:DOSEC(>2.0.ZU;2-6
Abstract
Aims: To study the incidence of Shiga-toxigenic Escherichia coli (STEC) in seafoods from India. Methods and Results: Escherichia coli isolated from various seafoods such a s fresh fish, clams and water were screened for the presence of stx, hlyA a nd rfb(O157) genes by PCR; 5% of clams and 3% of fresh fish samples were po sitive for non-(O157) STEC. Conclusions: STEC is prevalent in seafoods in India, and non-O157 serotype is more common. Significance and Impact of the Study: Seafood could be a vehicle for transm ission of STEC even in tropical countries.