Inflammatory cytokine levels are influenced by interactions between THP-1 monocytic, U-373 MG astrocytic, and SH-SY5Y neuronal cell lines of human origin

We measured the secretion of interleukin (IL)1 beta, IL-6 and tumor necrosi s factor-alpha (TNF-alpha )from human monocytic (THP-1), astrocytic (U-373 MG) and neuronal (SH-SY5Y) cell lines alone and in co-culture, with and wit hout stimulation by a combination of lipopolysaccharide (LPS) plus interfer on-gamma (IFN-gamma) or amyloid beta peptide 1-40 (A beta). LPS + IFN-gamma stimulation increased IL-1 beta secretion 16-fold from THP-1 cells. It inc reased IL-6 secretion 23-fold from THP-1 cells and 2.5-fold from U-373 MG c ells. It increased TNF-alpha secretion 3.4-fold from THP-1 cells, but did n ot influence its secretion from U-373 MG cells. It did not affect the secre tion of any of the cytokines from SH-SY5Ycells. A beta stimulation increase d IL-6 secretion 2.3-fold from U-373 MG cells but did not influence secreti on of IL-1 beta or TNF-alpha. A beta stimulation also failed to influence s ecretion of any of the cytokines from THP-1 or SH-SY5Y cells. When THP-1 an d U-373 MG cells were cocultured, IL-1 beta and IL-6 secretion, but not TNF -alpha secretion, were significantly reduced from the levels obtained in in dependent cultures, suggesting that a mutual suppressive action may occur b etween microglia and astrocytes. (C) 2001 Published by Elsevier Science Ire land Ltd.