A population study of the normal cornea using an in vivo, slit-scanning confocal microscope

Purpose. To document qualitative and quantitative changes in the normal, he althy human cornea with age using the confocal microscope. Methods. The cen tral corneas of 120 subjects (mean age, 41 years; range, 11 to 80 years) we re examined using an in vivo slit-scanning real-time confocal microscope. I mages of the corneal stroma and endothelium from both eyes of each subject were semiautomatically analyzed in an observer-masked, randomized manner. R esults. Anterior keratocyte density, posterior keratocyte density, and endo thelial cell density were shown to be unaffected by the sex of the subject with p values of 0.46, 0.55, 0.50, respectively (multivariate analysis of v ariance). No statistically significant difference was detected between righ t and left eyes for all corneal layers examined. The anterior keratocyte de nsity, posterior keratocyte density, and endothelial cell density decreased at a rate of 0.48, 0.22, and 0.33% per year, respectively. A positive corr elation was found between the coefficient of cell variation and age. Conclu sions. This data constitutes essential normative data that can be used as a control in further research into abnormal corneal conditions.