Effect of ethanol on tracheal potassium channels reconstituted into bilayer lipid membranes

We examined the effect of ethanol on single potassium channels derived from plasma membranes of bovine tracheal smooth muscles. The observed potassium channels had a conductance of 296 +/- 31 pS (mean +/-S.D.) in symmetrical 250 mmol/l KCl solutions, and exhibited a voltage- and Ca2+-dependence simi lar to BKCa channels. Ethanol at 50, 100 and 200 mM concentrations increase d the probability of open potassium channels to 112 +/-5, 127 +/-7 and 121 +/- 13% (mean +/-S.E.M.), respectively. It is suggested that increased acti vity of the BKCa channels by ethanol hyperpolarizes the plasma membrane and thus may contribute to relaxation of tracheal smooth muscle.