A reversed phase high performance liquid chromatography method for the analysis of boswellic acids in Boswellia serrata

An HPLC method for the separation of boswellic acids, the active constituen ts In Boswellia serrata resin has been developed. The first accurate determ ination of 6 individual acids was possible in the resin as well as in multi -component preparations. By using an acidic mobile phase, raised temperatur e and a 4 mum Synergi MAX-RP 80 Angstrom column the acids could be detected at levels as low as 0.9 mug/ml. The study of market products revealed sign ificant variations in the content of these pharmacologically active compoun ds in commercial samples.