The heterochromatic state of the Drosophila Y chromosome has made the cloni
ng and identification of Y-linked genes a challenging process. Here, we rep
ort application of a procedure to identify Y-linked gene fragments from the
unmapped residue of the whole genome sequencing effort, Previously identif
ied Y-linked genes appear in sequenced scaffolds as individual exons appare
ntly because many introns have become heterochromatic, growing to enormous
size and becoming virtually unclonable. A TBLASTN search using all known pr
oteins as query sequences, tested against a blastable database of the unmap
ped fragments, produced a number of matches consistent with this scenario.
Reverse transcription-PCR and genetic methods were used to confirm those th
at are expressed, Y-linked genes. The five genes reported here include thre
e protein phosphatases (Pp1-Y1, Pp1-Y2, and PPr-Y), an occludin-related gen
e (ORY), and a coiled-coils gene (CCY). This brings the total to nine prote
in-coding genes identified on the Drosophila Y chromosome. ORY and CCY may
correspond, respectively, to the fertility factors ks-1 and ks-2, whereas t
he three protein phosphatases represent novel genes. There remains a strong
functional coherence to male function among the genes on the Drosophila Y
chromosome.