Collection of Arabidopsis thaliana morphological insertion mutants

A collection of transgenic Arabidopsis thaliana plants has been obtained by Agrobacterium-mediated transformation. The genomes of the transgenic plant s contain insertions of T-DNA of the vector plasmids pLD3 or pPCVRN4. Genes bearing T-DNA insertions were shown to constitute 12-18% of the total numb er of A. thaliana genes. Seventy-five lines have been chosen from the colle ction and subjected to genetic and molecular-genetic analysis. Of these, 5 were dominant mutants, and 70, recessive insertion mutants with various mor phological defects. Identification of mutant phenotypes and genetic charact erization of the transgenic lines have been performed with the use of nutri ent media supplemented with exogenous hormones, which revealed five recessi ve lethal mutants and one dominant sterile mutant.