RAPD analysis of the genome in species of the genus Hordeum

Random amplification of polymorphic DNA (RAPD) was used to analyze six spec ies, three populations, and seven regional cultivars of barley. A unique pa ttern of amplified DNA products was obtained for each species of the genus Hordeum. High polymorphism of barley species was revealed. Specific fragmen ts were found in most RAPD patterns; the fragments can be used as molecular markers of corresponding species and subspecies. Several other DNA fragmen ts were shown to serve as molecular markers of the H genome. Specific RAPD patterns were obtained for each population and each cultivar of H. vulgare sensu lato. In total, variation between the populations and between the cul tivars was substantially lower than between species. Cluster analysis (UPGM A) was used to estimate genetic distances between the Hordeum species, betw een the H. spontaneum populations, and between regional H. vulgare cultivar s and a dendrogram was constructed.