Nb. Tomilova et al., Identification of a mutant gene controlling necrotic cotyledons in developing seedlings of Arabidopsis thaliana, RUSS J GEN, 37(4), 2001, pp. 392-400
Genetic and molecular analyses of an Arabidopsis thaliana mutant with necro
tic cotyledons from the collection of insertion mutants obtained earlier we
re conducted. The mutation under study showed incomplete dominance and repr
esented a single insertion of the T region of pLD3 vector used for transfor
mation of germinating seeds to the plant genome during the creation of the
collection. Using TAIL-PCR, a fragment of the mutant DNA adjacent to the le
ft border of the T-DNA insertion was isolated and sequenced. Computer r-aid
ed analysis showed that the insertion was located on the left arm of chromo
some 1. The open reading frame containing the insertion has one exon and en
codes a protein of 446 amino acids, whose function is unknown.