RAPD analysis of maize somaclones

The genetic difference between maize line A188 and A188-derived somaclones was assessed via analysis of randomly amplified polymorphic DNA (RAPD). In total, 17 decanucleotide primers used each allowed amplification of 2-17 fr agments ranging 200-2000 bp. The RAPD patterns did not differ between indiv idual plants of line A188, which demonstrated again its high genetic homoge neity. The difference between the initial line and the somaclones was high, ranging 64-74%. On evidence of the genetic divergence, the somaclones form ed two clusters. The distribution of somaclones between these clusters was consistent with their origin.