T. Forsstrom et al., Determination of potentially hallucinogenic N-dimethylated indoleamines inhuman urine by HPLC/ESI-MS-MS, SC J CL INV, 61(7), 2001, pp. 547-556
Citations number
23
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research General Topics
Journal title
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
A new method for the determination of N,N-dimethyl-5-hydroxytryptamine (buf
otenine), N,N-dimethyltryptamine (DMT)*, 5-methoxy-N,N-dimethyltryptamine (
5-MeODMT), and N-methyltryptamine (NMT) was developed using highperformance
liquid chromatography-mass spectrometry (HPLC-MS). Identification of the a
nalytes is based on liquid chromatographic retention times of analytes and
two fragment ions produced by a triple quadrupole mass spectrometer. Quanti
fication is based on electrospray ionization (ESI), and multiple reaction m
onitoring (MRM) was also utilized for getting better selectivity. The analy
tes and internal standard were separated from the urine matrix by solid-pha
se extraction (SPE). The method was applied for the determination of these
compounds in urine samples of patients from surgical. medical and psychiatr
ic wards. Of the dimethylated amines, only bufotenine was found in signific
ant amounts (up to 34 mug/L). In keeping with our earlier results. the bufo
tenine excretion of psychiatric patients was found to be higher than that o
f the somatic patients. Method, procedure, considerations, statistical eval
uations and urine sample spectra are presented.