EVALUATION OF A CHIRAL STATIONARY-PHASE BASED ON MIXED IMMOBILIZED PROTEINS

The preparation of a new chiral stationary phase (CSP) based on mixed immobilized human serum albumin (HSA) and alpha(1)-acid glycoprotein ( AGP) is reported. Enantiomeric separations of basic, acidic and neutra l compounds on this AGP-HSA CSP were compared to those obtained on a H SA CSP and on a AGP CSP. The results show that the new CSP has a wider range of applications than the initial CSPs. For two compounds, the e nantioselectivity on the mixed CSP was higher than on the HSA CSP.