Al. Simanov, HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF LOW-MOLECULAR-MASS PRODUCTS SYNTHESIZED BY POLYNUCLEOTIDE PHOSPHORYLASE IN POLYMERIZATION REACTION, Journal of chromatography, 685(1), 1994, pp. 39-44
HPLC was used for the simultaneous analysis of low- and high-molecular
-mass products of nucleotide polymerization. Oligonucleotides with cha
in lengths of less than 5 monomer units were found in the reaction mix
ture at the very beginning of the reaction. These oligomers were not t
he products of non-specific polymer degradation. An increase in NaCl c
oncentration from 0 to 1 IM resulted in the appearance of a short lag
period and the oligonucleotide accumulation. A simultaneous increase i
n Mg2+ concentration eliminated the lag. It is concluded that high ion
ic strength affects both the formation of the metal-monomer-polymer co
mplex and the affinity of the polynucleotide phosphorylase molecule fo
r the reaction products.