C. Tzavelas et al., Effect of osmolarity and presence of serum on the efficiency of cell transfection using immunoporation, ANAL CELL P, 22(4), 2001, pp. 223-227
Citations number
16
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Immunoporation is a novel method of cell transfection based upon the use of
a new type of beads, Immunofect beads, that can be targeted to make holes
in different types of cells depending on the type of bead used. It is known
that the efficiency of transfection of cells by some techniques can be aff
ected by the presence of serum and another important factor that appears to
affect transfection efficiency and cell viability is the osmolarity of the
transfection medium. This report presents studies on the effects of serum
and varying osmolarity on the efficiency of transfection using immunoporati
on. The results clearly indicate that in hypertonic media the presence of s
erum decreases the efficiency of transfection. In the case of osmolarity, i
ncreasing the osmolarity of the immunoporation medium increases the efficie
ncy of transfection but above about 650 mOsm this increasing efficiency is
offset by the much lower viability of the cells.