Characterization of a chromosomally encoded extended-spectrum class A beta-lactamase from Kluyvera cryocrescens

Authors
Decousser, JW Poirel, L Nordmann, P
Citation
Jw. Decousser et al., Characterization of a chromosomally encoded extended-spectrum class A beta-lactamase from Kluyvera cryocrescens, ANTIM AG CH, 45(12), 2001, pp. 3595-3598
Citations number
32
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
45
Issue
12
Year of publication
2001
Pages
3595 - 3598
Database
ISI
SICI code
0066-4804(200112)45:12<3595:COACEE>2.0.ZU;2-6
Abstract
A chromosomally located beta -lactamase gene, cloned and expressed in Esche richia coli from a reference strain of the enterobacterial species Kluyvera cryocrescens, encoded a clavulanic acid-inhibited Ambler class A enzyme, K LUC-1, with a pl value of 7.4. KLUC-1 shared 86% amino acid identity with a subgroup of plasmid-mediated CTX-M-type extended-spectrum P-lactamases (CT X-M-1, -3, -10, -11, and -12), the most closely related enzymes, and 77% am ino acid identity with KLUA-1 from Kluyvera ascorbata. The substrate profil e of KLUC-1 corresponded to that of CTX-M-type enzymes.