Expression of green fluorescent protein as a marker for effects of antileishmanial compounds in vitro

Authors
Kamau, SW Grimm, F Hehl, AB
Citation
Sw. Kamau et al., Expression of green fluorescent protein as a marker for effects of antileishmanial compounds in vitro, ANTIM AG CH, 45(12), 2001, pp. 3654-3656
Citations number
24
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
45
Issue
12
Year of publication
2001
Pages
3654 - 3656
Database
ISI
SICI code
0066-4804(200112)45:12<3654:EOGFPA>2.0.ZU;2-2
Abstract
Transgenic Leishmania infantum promastigotes, which constitutively express green fluorescent protein (GFP) in their cytoplasm, were used to monitor th e effects of antileishmanial compounds in real time. The GFP-based assay pr ovided a reliable measure of drug-induced inhibitory effects on protein exp ression, resulting in a dynamic picture of the responses of leishmanial pro mastigotes to the compounds tested.