Targetting AFLP-DNA markers to specific traits and chromosome regions

The amplified fragment length polymorphism (AFLP) technique is widely used in mapping of wheat as high polymorphism rates are obtained and the procedu re is relatively simple. In this study the AFLP markers were targetted to w heat chromosome regions of interest, especially those in which random mappi ng approaches located relatively few markers. Results showed that the combi nation of bulk segregant analysis and AFLP markers could target new markers to regions of interest in wheat, and as a result, additional markers were identified for the dough mixing time and noodle colour traits. The new mark ers for noodle colour were closer to this trait than the markers previously identified by random procedures. As a result, their association with the t rait was more significant, an aspect that is important for selection effici ency. In order to improve genome coverage, it was found that regions of chr omosomes containing telomere sequences could be targetted using AFLPs combi ned with a telomere sequence anchor primer.