Albumin polymorphism and mapping of a dimeric alpha-amylase inhibitor in wheat

Any new protein or DNA marker is potentially useful to add detail to alread y constructed genetic chromosome maps and may be valuable in breeding progr ams wherever polymorphism exists. Non-gluten proteins represent 15-20% of t otal wheat grain proteins. Isoelectric focusing of wheat (Triticum aestivum L. em Thell.) proteins on ultrathin gels showed high resolution and was fo und to be a useful tool in the differentiation of wheat varieties. Seventee n hexaploid wheat varieties were screened to investigate polymorphism of al bumin proteins using isoelectric focusing. Polymorphism was observed for al bumin polypeptides of pI 5.20, 5.85, 6.25, and 7.1, and 8.0. The polymorphi c protein of pI 7.1 was mapped by analysing doubled haploid populations fro m the intervarietal crosses, Cranbrook x Halberd and Synthetic x Opata 85. This protein locus was designated as Iha-B1.2, and is located on the short arm of chromosome 3B.