First images of a glutamate receptor ion channel: Oligomeric state and molecular dimensions of GluRB homomers

We have expressed, purified, and characterized glutamate receptor ion chann els (GluR) assembled as homomers of the subunit GluRB. For the first time, single-milligram quantities of biochemically homogeneous GluR have been obt ained. The protein exhibits the expected pharmacological profile and a high specific activity for ligand binding. Density-gradient centrifugation reve als a uniform oligomeric assembly and a molecular mass suggesting that the channel is a tetramer. On the basis of electron microscopic images, the rec eptor appears to form an elongated structure that is visualized in several orientations. The molecular dimensions of the molecule are approximately 11 x 14 x 17 nm, and solvent-accessible features can be seen; these may contr ibute to formation of the ion-conducting pathway of the channel. The channe l dimensions are consistent with an overall 2-fold symmetric assembly, sugg esting that the tetrameric receptor may be a dimer of dimers.