Production of Flammulina velutipes on coffee husk and coffee spent-ground

Solid state cultivation (SSC) was carried out to evaluate the feasibility o f using coffee husk and spent-ground as substrates for the production of ed ible mushroom Flammulina under different conditions of moisture and spawn r ate. The strain of F. velutipes LPB 01 was adapted for a coffee husk extrac t medium. Best results were obtained with 25% spawn rate, though there was not much difference when lower spawn rates (10-20%) were used. Ideal moistu re content for mycelial growth was 60% and 55% for coffee husk and spent-gr ound, respectively. With coffee husk as substrate, first fructification occ urred after 25 days of inoculation and the biological efficiency reached ab out 56% with two flushes after 40 days. With spent-ground as substrate, fir st fructification occurred 21 days after inoculation and the biological eff iciency reached about 78% in 40 days. There was decrease in the caffeine an d tannins contents (10.2 and 20.4%, respectively) in coffee husk after 40 d ays. In coffee spent-ground, the tannin contents decreased by 28% after 40 days. These decrease was attributed to the degradation of caffeine or tanni ns by the culture because these were not adsorbed in the fungal mycelia. Re sults showed the feasibility of using coffee husk and coffee spent-ground a s substrate without any nutritional supplementation for cultivation of edib le fungus in SSC. Spent ground appeared better than coffee husk.