Iv. Broek et al., Laminin-1-induced migration of multiple myeloma cells involves the high-affinity 67 kD laminin receptor, BR J CANC, 85(9), 2001, pp. 1387-1395
The 67 kD laminin receptor (67LR) binds laminin-1 (LN), major component of
the basement membrane, with high affinity. In this study, we demonstrated t
hat human multiple myeloma cell lines (HMCL) and murine 5T2MM cells express
67LR. CD38(bright+), plasma cells in fresh multiple myeloma (MM) bone marr
ow (BM) samples showed weaker 67LR expression, but expression increased aft
er direct exposure to a BM endothelial cell line (4LHBMEC). LN stimulated t
he in vitro migration of 3 HMCL (MM5.1, U266 and MMS.1), primary MM cells a
nd the murine 5T2MM cells. 67LR has been shown to mediate the actions of LN
through binding to CDPGYIGSR, a 9 amino acid sequence from the B1 chain of
LN. MM cell migration was partially blocked by peptide 11, a synthetic non
apeptide derived from this amino sequence and also by a blocking antiserum
against 67LR. Co-injection of peptide 11 with 5T2MM cells in a murine in vi
vo model of MM resulted in a decreased homing of 5T2MM cells to the BM comp
artment. In conclusion, LN acts as a chemoattractant for MM cells by intera
ction with 67LR. This interaction might be important during extravasation o
f circulating MM cells. (C) 2001 Cancer Research Campaign.