Mechanisms involved in alpha 6 beta 1-integrin-mediated Ca2+ signalling

Contact of Jurkat T-lymphocytes with the extracellular matrix (ECM) protein laminin resulted in long-lasting alpha6 beta1-integrin-mediated Ca2+ signa lling. Both Ca2+ release from thapsigargin-sensitive Ca2+ stores and capaci tative Ca2+ entry via Ca2+ channels sensitive to SKF 96365 constitute impor tant parts of this process. Inhibition of alpha6 beta1-integrin-mediated Ca 2+ signalling by (1) the src kinase inhibitor PP2, (2) the PLC inhibitor U7 3122, and (3) the cyclic adenosine diphosphoribose (cADPR) antagonist 7-dea za-8-Br-cADPR indicate the involvement of src tyrosine kinases and the Ca2-releasing second messengers D-myo-inositol 1.4,5-trisphosphate (InSP3) and cADPR. (C) 2001 Elsevier Science Inc. All rights reserved.