DIFFERENCES IN THE EXPRESSION OF GLUCOSE-TRANSPORTER PROTEIN ISOFORMSIN HUMAN RETINOBLASTOMA CELL-LINES

We investigated the expression of glucose transporter (GLUT) protein i soforms in two human retinoblastoma cell Lines, Y79 and WERI-Rb1, by W estern blotting analysis with anti-GLUT1,2,3, and 4 antibodies. GLUT1 and GLUT4 proteins were detected in Y79, whereas GLUT1 and GLUT3 prote ins were found in WERI-Rb1. GLUT2 protein was not detected in Y79 or W ERI-Rb1. Our findings are of interest because (1) the expression of GL UT protein isoforms in the two retinoblastoma cell lines was different , and (2) GLUT4, protein, the insulin-sensitive GLUT isoform, was dete cted in Y79. This suggests that these cell lines have different mechan isms of glucose transport. (C) 1997 Japanese Ophthalmological Society.