Studies on adsorption of TNF alpha with macroporous resin

There are increasing evidences that high serum levels of Tumor Necrosis Fac tor (TNF alpha) have been shown to be associated with disease severity in f ulminant hepatic failure and dyscrasia, which is a principal mediator in th e cascade of pathophysioligic events following endotoxic shock. In the pres ent study, NK-110, carbonized resin and MET-1004 are selected as adsorbents in the static adsorption experiments of TNF alpha. We have investigated th e adsorption capacity, kinetic profiles and adsorption isotherm of the thre e macroporous resins. Experimental results show that MET-1004 and NK-110 ha ve higher adsorption capacities than carbonized resin. Compared with NK-110 , the velocity of adsorption of MET-1004 towards TNF alpha is much faster t han the former. From experimental data the adsorption rate constants k are calculated to be 0.016 3 min(-1) (NK-110); 0.018 9 min(-1) (carbonized resi n); 0.007 9 min(-1) (MET-1004) at 37 degreesC, respectively. Furthermore, t heir adsorption isotherms are also in different shapes. They are shown to b e "H"(MET-1004); "L"(NK-110); "S" (carbonized resin) at 37 degreesC, respec tively. This demonstrated that the surface-to-volume ratio and the pore siz e of macroporous resins had an strong impact on the adsorption capacity and as the pore size increased, the adsorptive rate increased.