Nuclear magnetic resonance studies on the effects of Rare Earth in rats urine

Male Wistar rats were administrated orally with La(NO3)(3) at doses of 0. 0 5, 0. 2, 2. 0, 10 and 20 mg/kg body weight. Urine was collected over a 24 h period after dosing. Resonances for a large number of low molecular weight metabolites were assigned in a high resolution H-1 NMR spectra of rat urin e. The variation of some low molecular weight metabolites in urine provided a sensitive measurement of Rare Earth induced renal and liver lesions, in which DMA, DMG, urea, Kg, TMAO, succinate, citrate and amino acids have bee n suggested as NMR markers for renal damage and ethanol, lactate, taurine a s the markers for liver damage. The method could be applicable to study of the toxicological effects of other compounds and drugs.