DNA is of structural polymorphism, which is useful in nanoarchitecture; esp
ecially, four-arm DNA junctions can be used to assemble nanowebs. The stati
c four-arm DNA junctions were designed and synthesized. One-arm DNA and two
-arm DNA came out simultaneously with the four-arm DNA junction's formation
. A new method, termed the two-step method, was proposed and the productivi
ty of four-arm DNA junctions was Increased. A nanoweb was assembled success
fully, but it showed irregularity itself. It was not the same as we expecte
d. We consider that it is a result from the flexibility of four-arm DNA jun
ction.