Cloning and functional research of renal cell carcinoma related novel gene- GYLZ-RCC18

After the renal cell carcinoma related novel gene fragment GYLZ-RCC18 was c loned by using suppression subtractive hybridization (SSH), we used the SMA RT RACE technology to clone the full length of GYLZ-RCC18 and performed chr omosome location by the FISH method. RT-PCR was used to detect the expressi on of the first reading frame of GYLZ-RCC18 in different stages and grades of renal cell carcinoma tissue and other tissues. Also we transfected the a ntisense oligonucleotide of GYLZ-RCC18 to renal cell carcinoma cell line GR C-1, and analyzed the proliferation activity, growth speed, apoptosis and m ortality changes in GRC-1. The results show that the full length of GYLZ-RC C18 (GenBank accession No.: BE825133) cDNA is about 3.5 kb long which is lo cated at No. 14 chromosome. GYLZ-RCC18 has a higher expression in higher gr ades and stages of renal cell carcinoma than in the lower ones. The express ion of GYLZ-RCC18 in renal cell carcinoma was much higher than that in norm al kidney and other tissues. After transfection of GYLZ-RCC18 antisense oli gonucleotide, the mortality of GRC-1 increases evidently, the proliferation activity and growth speed were inhibited remarkably at the same time. Also the antisense oligonucleotide can induce the apoptosis of GRC-1 all throug h the observation time. Our results indicated that GYLZ-RCC18 is an importa nt novel gene related to renal cell carcinoma. Its overexpression would sti mulate the growth and proliferation activity and plays an antidead and anti apoptosis effect in renal cell carcinoma. Transfection of antisense oligonu cleotide could inhibit the generation and development of renal cell carcino ma. The study provides a new clue for the research of renal cell carcinoma, and also provides an instruction for special genetic diagnosis and the the rapy of renal cell carcinoma.