Genotyping of Anatolian doubled-haploid durum lines with SSR markers

In this study, doubled haploid lines generated from the durum wheat varieti es, selections from Middle Anatolian landraces, 'Cakmak-79', 'Berkmen-469' and 'Kunduru-1149' (Savaskan et al., 1997), were analyzed using ten highly polymorphic microsatellite markers for genotyping and evaluation of genetic relationships between and within the doubled-haploid (DH) lines. The avera ge PIC value was found to be 0.531. Populations of doubled-haploid lines of landrace selected cultivars 'Cakmak-79' and 'Kunduru-1149', were the two m ost distant populations with (delta mu)(2) = 1.42. 'Berkmen-469' x 'Cakmak- 79' and 'Berkmen-469' x 'Kunduru-1149' yielded similar genetic distances, ( delta mu)(2) of 0.84 and 0.85, respectively. In addition, the genetic relat ionship between the progenitors of the DH lines together with other durum w heat varieties was analyzed. A meaningful relationship was obtained based o n available pedigree information on the cultivars.