STUDIES ON THE MECHANISM OF FLUORESCENCE QUENCHING OF TB3-TIRON COMPLEX BY NUCLEOTIDES, POLYNUCLEOTIDES AND NUCLEIC-ACIDS()

The mechanism of fluorescence quenching of Tb3+-Trion complex by nucle otides, polynucleotides and nucleic acids has been studied, It was con sidered that it is a static quenching which is based on the phosphate moieties of nucleotides, polynucleotides and nucleic acids conpeting t he Tb3+ ion with Tiron and forming non-fluorescent binary complex unde r the optimal conditions, The experimental results of nucleic acids re acting with intercalating reagent of DNA or metal ion using Tb3+-Trion complex as a fluorescence probe indicated that the mechanism is reaso nable.