INVESTIGATION ON THE SEPARATION OF MALTOO LIGOSACCHARIDE BY CAPILLARYZONE ELECTROPHORESIS

The effect of ionic hydrodynamic radius in the separation of capillary zone electrophoresis is discussed by using maltooligosaccharide as mo del compounds. The linear correlation between mu(cp) and n(-1) (n is t he number containing the glycosyl units) has been derived and has expe rimentally been verified with various buffer systems and separation vo ltages. The results show that hydrodynamic radius is one of the actual factors in HPCZE other than molecular weights. The proposed equation could provide the approach determining glycosyl units of maltooligosac charide.