In silico screening for tumour-specific expressed sequences in human genome

A computer-based differential display tool named HsAnalyst has been develop ed and successfully used for the comparison of expression patterns in a set of tumours versus a set of normal tissues. A list of EST clusters highly r epresented in tumours and rarely observed in normal tissues has been develo ped as a resulting output rile of the program. These differentially express ed EST clusters (genes) can be useful for developing new tumour markers and prognostic indicators for a wide set of human malignancies. Tumour-specifi c protein-coding genes may be considered a manifestation of tumour-specific gene expression. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.