Two sequence classes of kinetoplastid 5S ribosomal RNA gene revealed amongbodonid spliced leader RNA gene arrays

The spliced leader RNA genes of Bodo saltans, Cryptobia helicis and Dimasti gella trypaniformis were analyzed as molecular markers for additional taxa within the suborder Bodonina. The non-transcribed spacer regions were disti nctive for each organism, and 5S rRNA genes were present in Bodo and Dimast igella but not in C helicis. Two sequence classes of 5S rRNA were evident f rom analysis of the bodonid genes. The two classes of 5S rRNA genes were fo und in other Kinetoplastids independent of co-localization with the spliced leader RNA gene. (C) 2001 Federation of European Microbiological Societies . Published by Elsevier Science B.V. All rights reserved.