Envm. Carrilho et al., An attempt to correlate fat and protein content of biological samples withresidual carbon after microwave-assisted digestion, FRESEN J AN, 371(4), 2001, pp. 536-540
The residual carbon content of a variety of bovine-derived samples and fora
ge was determined by inductively coupled plasma optical emission spectromet
ry with radial view configuration (ICP-OES) after microwave-assisted digest
ion under high pressure in a closed vessel. The original carbon concentrati
on in the samples was determined by elemental analysis. The highest amount
of original carbon content (64%) was found in viscera. After digestion, up
to 75% of it was destroyed. Viscera presented the highest ether extract and
blood exhibited a high crude protein content of up to 99%. The efficiency
in destroying the organic matter in biological materials seemed to be relat
ed to their fat content and showed no significant difficulty for protein-ri
ch samples. The correlation coefficient between the fat content of the samp
les and the residual carbon after acid decomposition was 0.9173 indicating
a fair fit. However, no correlation was observed between % RC and the prote
in content.