Intra-species DNA polymorphism in the tobacco cyst-nematode complex (Globodera tabacum) using AFLP

Amplified fragment length polymorphism (AFLP) was used to obtain informatio n on the within-species genetic variability of the tobacco cyst - nematode (TCN) complex. AFLP was found to be well suited to this type of study. The current classification of TCN was confirmed. Results indicate that the Glob odera tabacum solanacearum group, believed to be restricted to the U.S.A., also occurs in Mexico. The within-species variability of TCN is considerabl e. Populations from Mexico may form a new subgroup. AFLP group-specific mar kers were identified for two of the TCN subgroups: Globodera tabacum tabacu m and Globodera tabacum solanacearum.