Jc. Glaubitz et al., Dinucleotide microsatellites from Eucalyptus sieberi: inheritance, diversity, and improved scoring of single-base differences, GENOME, 44(6), 2001, pp. 1041-1045
Eight dinucleotide microsatellites were developed in Eucalyptus sieberi L.
Johnson (silvertop ash), a member of the subgenus Eucalyptus. Transfer of s
ix of these to the subgenus Symphyomyrtus and their Mendelian inheritance a
re demonstrated using a full-sib cross in Eucalyptus nitens. Genetic divers
ity parameters are presented for the eight loci based on a sample of 100 ol
d-growth E. sieberi trees from a single natural stand. One locus, Es266, ha
d an atypically high fixation index, and significantly deviated from Hardy-
Weinberg equilibrium genotypic proportions, indicating the likely presence
of null alleles. Two of the loci, Es076 and Es140, had many alleles that di
ffered in size by only a single base pair, possibly because of short poly(A
) or poly(T) stretches in their flanking regions. These two loci were by fa
r the most polymorphic, but were difficult to score reliably on a capillary
DNA sequencer. Reliability of scoring of these two one-base microsatellite
loci was markedly improved by the incorporation of internal reference alle
les into each sample analysed.