Quantitative analysis of two-dimensional gel electrophoresis protein patterns: a method for studying genetic relationships among Globodera pallida populations
A. Fullaondo et al., Quantitative analysis of two-dimensional gel electrophoresis protein patterns: a method for studying genetic relationships among Globodera pallida populations, HEREDITY, 87, 2001, pp. 266-272
A method based in two-dimensional protein gel electrophoresis has been deve
loped in order to improve the analysis of genetic relationships among popul
ations of Globodera. It has been used to estimate genetic divergence among
nine Globodera pallida nematode populations. Sixty-one anonymous. polypepti
de spots were resolved using silver-stained high-resolution 2D gels and the
y were quantified in each population to establish genetic variation among G
. pallida populations. The results of this analysis were compared with thos
e obtained after a study of allelic frequency variation, which was carried
out using seven previously described loci. Genetic distances among populati
ons were calculated by means of both studies, the quantitative analysis and
the allelic frequency variation, and phylogenetic trees were constructed f
or each type of analysis. A correlation analysis between the two distance m
atrices was, carried out and a bootstrap analysis was performed to determin
e the strength of the clusters obtained with each method. The results obtai
ned support the idea that quantitative protein analysis can be successfully
applied to phylogenetic analysis of G. pallida populations.