Salmonella enterica serovar Pullorum persists in splenic macrophages and in the reproductive tract during persistent, disease-free carriage in chickens
P. Wigley et al., Salmonella enterica serovar Pullorum persists in splenic macrophages and in the reproductive tract during persistent, disease-free carriage in chickens, INFEC IMMUN, 69(12), 2001, pp. 7873-7879
Salmonella enterica serovar Pullorum is worldwide a poultry pathogen of con
siderable economic importance, particularly in those countries with a devel
oping poultry industry. In addition to the characteristic high mortality ra
tes among young chicks, one of the features of Salmonella serovar Pullorum
infection is that it persists for long periods in convalescent chicks in th
e absence of clinical disease. This can lead to colonization of the reprodu
ctive tract of chickens and at sexual maturity can result in infected proge
ny through transovarian transmission to eggs. The sites of Salmonella serov
ar Pullorum persistence in convalescent birds are not known, and the mechan
isms of persistence are not understood. Here we show that Salmonella serova
r Pullorum can persist in both the spleen and the reproductive tract for ov
er 40 weeks following experimental infection in chickens. During the period
of sexual maturity, Salmonella serovar Pullorum colonized both the ovary a
nd the oviduct of hens and led to 6% of laid eggs being infected by Salmone
lla serovar Pullorum. The colonization of several different sites of the re
productive tract suggests that Salmonella serovar Pullorum may employ more
than one mechanism of egg infection. Persistence occurred despite a strong
humoral response, suggesting an intracellular site of infection. By use of
a Salmonella serovar Pullorum strain containing a plasmid stably expressing
green fluorescent protein, we demonstrated that the main site of carriage
in the spleen is within macrophages. This raises interesting questions abou
t the biology of Salmonella serovar Pullorum, including why there is an inc
rease in bacterial numbers when birds become sexually mature and in particu
lar how Salmonella serovar Pullorum avoids clearance by macrophages and whe
ther it modulates the immune system In other ways.