pH-sensitive nanoparticles of poly(amino acid) dodecanoate complexes

Nanoparticles were formed by the complexation of poly(L-arginine) (PLA), po ly(L-histidine) (PLH) and poly(L-lysine) (PLL) with dodecanoic acid (C12). Dynamic light scattering, potential measurements, atomic force microscopy, fluorescence, and circular dichroism spectroscopy were used for their chara cterization. It was found that the diameters of the poly(L-arginine) dodeca noate (PLA-C12), poly(L-histidine) dodecanoate (PLH-C12), and poly(L-lysine ) dodecanoate (PLL-C12) complex nanoparticles were in the range 120-200 nm. Furthermore, the pH-sensitive dissolution and the surface charges can be a djusted by choosing PLA, PLH and PLL. The particle stability against basic pH values increases with increasing pK(a) value of the poly(amino acid) in the series PLH-C12, PLL-C12 and PLA-C12. The particles as such show a core- shell morphology. Their cores are formed by stoichiometric poly(amino acid) dodecanoate complexes while the shells stabilizing the particles are forme d by cationic poly(amino acid) chains in an uncomplexed state. The particle s were tested as containers for hydrophobic molecules such as pyrene, which served as a fluorescence probe for measuring the polarity within the parti cles, and Q(10) which functioned as a model drug. The maximum uptake of Q(1 0) into the nanoparticles is about 13% (w/w), thereby making the complexes attractive as simple drug carriers for controlled release purposes. Circula r dichroism measurements revealed that the poly(amino acid) chains of PLA-C 12 and PLL-C12 adopt predominantly an alpha -helix and that of PLH-C12 a be ta -sheet. (C) 2001 Elsevier Science B.V. All rights reserved.