Four strains of a hitherto unrecognized Gram-positive, catalase-negative, f
acultatively anaerobic, rod-shaped bacterium isolated from human sources we
re characterized using phenotypic and molecular taxonomic methods. Comparat
ive 16S rRNA gene sequencing demonstrated that the bacterium represents a n
ew subline within the Lactobacillus casei/Pediococcus rRNA group of the gen
us Lactobacillus. The unknown bacterium was readily distinguished from all
other described Lactobacillus species and related taxa by biochemical tests
and electrophoretic analysis of whole-cell proteins. On the basis of phylo
genetic and phenotypic evidence, it is proposed that the unknown bacterium
be classified as Lactobacillus coleohominis sp. nov. The type strain of Lac
tobacillus coleohominis is CCUG 44007(T) (= CIP 106820(T)).