Metabolism of cinnamic acids by some Clostridiales and emendation of the descriptions of Clostridium aerotolerans, Clostridium celerecrescens and Clostridium xylanolyticum
M. Chamkha et al., Metabolism of cinnamic acids by some Clostridiales and emendation of the descriptions of Clostridium aerotolerans, Clostridium celerecrescens and Clostridium xylanolyticum, INT J SY EV, 51, 2001, pp. 2105-2111
Citations number
31
Categorie Soggetti
Microbiology
Journal title
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
The ability of Clostridium aerotolerans DSM 5434(T), Clostridium celerecres
cens DSM 5628(T), Clostridium methoxybenzovorans DSM 12182(T), Clostridium
stercorarium ATCC 35414(T), Clostridium subterminale DSM 2636, Clostridium
termitidis DSM 5398(T), Clostridium thermolacticum DSM 2910(T), Clostridium
thermopalmarium DSM 5974(T) and Clostridium xylanolyticum DSM 6555(T) to m
etabolize cinnamic acid and various derivatives, with or without glucose su
pplementation, was examined. Only C. aerotolerans DSM 5434T and C. xylanoly
ticum DSM 6555T, closely related species, transformed cinnamic acid to 3-ph
enylpropionic acid. Both species also reduced a wide range of cinnamic acid
derivatives, including o-, m- and p-coumaric, o-, m- and p-methoxycinnamic
, p-methylcinnamic, caffeic, ferulic, isoferulic and 3,4,5-trimethoxycinnam
ic acids to their corresponding 3-phenylpropionic acid derivatives. C. aero
tolerans DSM 5434T, however, also decarboxylated p-coumaric acid into 4-vin
ylphenol, which was then reduced to 4-ethylphenol. C. celerecrescens was gr
ouped with C. aerotolerans and C. xylanolyticum in subcluster XIVa of the C
lostridiales. C. celerecrescens DSM 5628T only metabolized m- and p-methoxy
cinnamic and p-methylcinnamic acids to their corresponding 3-phenylpropioni
c acid derivatives, reducing the double bond in the C-3 aliphatic side chai
r. Addition of glucose markedly increased the yield of the biotransformatio
ns by these three species. An emendation of the descriptions of C. aerotole
rans, C. celerecrescens and C. xylanolyticum is proposed, based on these ob
servations.