Pseudomonas sp. strain KC (= ATCC 55595 = DSM 7136) is a denitrifying aquif
er isolate that produces and secretes pyridine-2,6-bis(thiocarboxylate) (PD
TC), a chelating agent that fortuitously transforms carbon tetrachloride wi
thout producing chloroform. Although KC has been used successfully for full
-scale bioremediation of carbon tetrachloride, its taxonomy has proven diff
icult to resolve, as it retains properties of both Pseudomonas stutzeri and
Pseudomonas putida. In the present work, a polyphasic approach was used to
conclude that strain KC represents a new genomovar (genomovar 9) within th
e species P. stutzeri.