OXA-35 is an OXA-10-related beta-lactamase from Pseudomonas aeruginosa

Pseudomonas aeruginosa clinical isolate PA35 is resistant to amino- and ure ido-penicillins, has intermediate susceptibility to cefsulodin, cefepime an d aztreonam, and is susceptible to imipenem and ceftazidime. Cloning and se quencing revealed a new,6-lactamase variant, OXA-35, sharing 96% amino acid identity with OXA-10. OXA-35 displays a restricted-substrate hydrolysis pr ofile with improved hydrolysis of amoxicillin and cloxacillin compared with OXA-10. OXA-35 differs from derivatives OXA-19 and OXA-28 by one amino aci d substitution and may be a progenitor of these OXA-13-like extended-spectr um,beta -lactamases.